pcr based diagnostic methods diabetes

pcr based diagnostic methods diabetes

Highly sensitive diagnosis of 43 monogenic forms of diabetes ... - NCBI

16 Sep 2013 ... Highly sensitive diagnosis of 43 monogenic forms of diabetes or obesity through one-step PCR-based enrichment in ... to develop innovative cost-efficient methods for sensitive diabetes and obesity multigene screening.PCR has now become a standard diagnostic and research tool in periodontology . .... to amplify RNA using RT-PCR facilitates laboratory-based diagnostic testing to ... pregnancy complications, diabetes, chronic kidney disease, osteoporosis, ...10 Sep 2013 ... All of these monogenic forms of diabetes and obesity tend to occur at younger ... assess a new method based on PCR enrichment in microfluidic droplets and .... Currently, the methods of molecular diagnosis based on Sanger ...A PCR test based on insertion sequence IS1081 was developed to detect ... to AIDS or to conditions such as alcohol abuse, renal disorders, diabetes mellitus, etc. ... of the blood-based PCR assay for the diagnosis of tuberculosis, especially in ...Two bacterial detection methods were used: (1) standard microbiological culturing, and (2) polymerase chain reaction (PCR) based diagnostic method to detect ...Highly sensitive diagnosis of 43 monogenic forms of diabetes or obesity, through one step PCR-based enrichment in combination with next generation sequencing ... Methods. We designed a PCR based sequence enrichment panel targeting ...The molecular diagnosis of monogenic forms of diabetes and obesity is crucial as ... This method is based on PCR in lipid microdroplets (RainDance technology, ...17 Jul 2014 ... ... droplet-based digital PCR (ddPCR) diabetes diagnostic. Islet holds an exclusive license to the epigenetic test, which detects methylation ...Sep 16, 2013 ... Highly sensitive diagnosis of 43 monogenic forms of diabetes or obesity through one-step PCR-based enrichment in ... to develop innovative cost-efficient methods for sensitive diabetes and obesity multigene screening.Aug 15, 2014 ... Molecular biology based diagnostic procedures for detecting bacteria in ... The use of the PCR-based method was effective for high-sensitivity detection ..... His current research activities focus on fracture healing in diabetes ...


However, standard culture methods may not be very effective for detecting orthopaedic infections [ Previous studies in our laboratory and others have demonstrated the superior sensitivity and accuracy of molecular biological technology, based on the DNA amplification technique of polymerase chain reaction (PCR); [ ]) and for the detection of infection in symptomatic arthroplasty patients (Mariani et al. Ten-fold serial dilutions of template DNA were used to generate the standard curve. Bereza PL, Ekiel A, Augusciak-Duma A, Aptekorz M, Wilk I, Kusz DJ, Wojciechowski P, Martirosian G. In all cases, except for rabbit #7, positive control product was detected from the extracts. Only 1 of 4 animals gave a positive bacteriological culture test; in fact, of the 16 specimens obtained with the different biopsy methods, only 1 test was positive.

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